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TMF1-Specific Polyclonal antibody

TMF1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 19728-1-AP

Print datasheet

Synonyms

TMF1, TMF, TATA element modulatory factor, ARA160, Androgen receptor-associated protein of 160 kDa

验证数据展示

  • IHC staining of human cervical cancer using 19728-1-AP

    IHC staining of human cervical cancer using 19728-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMF1 antibody included
  • IHC staining of human cervical cancer using 19728-1-AP

    IHC staining of human cervical cancer using 19728-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMF1 antibody included
  • WB analysis using 19728-1-AP

    WB analysis using 19728-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19728-1-AP (TMF1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 19728-1-AP

    IP experiment of HepG2 using 19728-1-AP

    IP result of anti-TMF1-Specific (IP:19728-1-AP, 4ug; Detection:19728-1-AP 1:300) with HepG2 cells lysate 3600ug.

  • IF Staining of HepG2 using 19728-1-AP

    IF Staining of HepG2 using 19728-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inBxPC-3 cells, L02 cells, Hela cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman cervical cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TMF1 Ready-To-Use IHC Kit. TMF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19728-1-AP targets TMF1-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TATA element modulatory factor 1
Synonyms TMF1, TMF, TATA element modulatory factor, ARA160, Androgen receptor-associated protein of 160 kDa
Calculated Molecular Weight 123 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberNM_007114
Gene Symbol TMF1
Gene ID (NCBI) 7110
RRIDAB_10667009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP82094
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.

实验方案

Product Specific Protocols
WB protocol for TMF1-Specific antibody 19728-1-APDownload protocol
IHC protocol for TMF1-Specific antibody 19728-1-APDownload protocol
IF protocol for TMF1-Specific antibody 19728-1-APDownload protocol
IP protocol for TMF1-Specific antibody 19728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Mol Cell

An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.

Authors - Julian Nüchel
View Article
WB

J Biol Chem

Discovery Proteomics Identifies a Molecular Link between the Coatomer Protein Complex I and Androgen Receptor-dependent Transcription.

Authors - Jordy J Hsiao
View Article