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验证数据展示

  • WB analysis of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

    WB analysis of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

    IHC staining of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

    IHC staining of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

  • WB analysis using 27174-1-AP (same clone as 27174-1-PBS)

    WB analysis using 27174-1-AP (same clone as 27174-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

TMIGD1 Polyclonal antibody, PBS Only

TMIGD1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27174-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

TMIGD, Transmembrane and immunoglobulin domain-containing protein 1, UNQ9372/PRO34164

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

27174-1-PBS targets TMIGD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 TMIGD1 fusion protein Ag25691 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane and immunoglobulin domain containing 1
别名 TMIGD, Transmembrane and immunoglobulin domain-containing protein 1, UNQ9372/PRO34164
计算分子量 262 aa, 29 kDa
观测分子量 45 kDa, 29 kDa
GenBank蛋白编号BC137201
基因名称 TMIGD1
Gene ID (NCBI) 388364
RRIDAB_2880786
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXZ0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMIGD1 (Transmembrane and immunoglobulin domain containing 1) is involved in various cellular processes such as regulation of cell structure, cell-cell adhesion, and cellular movement. It protects cells from oxidative- and nutrient-deprivation-induced cell injury by stabilizing the transepithelial electric resistance and permeability of renal epithelial cells. TMIGD1 has a calculated molecular mass of 29 kDa, and can be detected as 45 kDa due to the N-glycosylation (PMID: 26342724).