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TMPRSS2 Monoclonal antibody

TMPRSS2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H7E9

Cat No : 68264-1-Ig

Print datasheet

Synonyms

FLJ41954, PP9284, PRSS10, Serine protease 10, TMPRSS2

验证数据展示

  • WB analysis using 68264-1-Ig

    WB analysis using 68264-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68264-1-Ig

    WB analysis using 68264-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of pig kidney using 68264-1-Ig

    WB analysis of pig kidney using 68264-1-Ig

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPC-3 cells, pig kidney tissue, LNCaP cells, Caco-2 cells, COLO 320 cells, HT-29 cells, human testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68264-1-Ig targets TMPRSS2 in WB, ELISA applications and shows reactivity with Human, Pig samples.

Tested Applications WB, ELISA
Tested Reactivity Human, Pig
Immunogen TMPRSS2 fusion protein Ag5824 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transmembrane protease, serine 2
Synonyms FLJ41954, PP9284, PRSS10, Serine protease 10, TMPRSS2
Calculated Molecular Weight 54 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC051839
Gene Symbol TMPRSS2
Gene ID (NCBI) 7113
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15393
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMPRSS2, also named as PRSS10, is a type II transmembrane serine protease which is highly expressed by the epithelium of the human prostate gland. TMPRSS2 may contribute to prostate tumour metastasis via the activation of PAR-2. TMPRSS2 is a Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. TMPRSS2 is a host cell factor that is critical for the spread of several clinically relevant viruses, including influenza A viruses and coronaviruses (PMID: 23468491, 30626688). SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. The initial spike protein priming by TMPRSS2 is essential for the entry and viral spread of SARS-CoV-2 through interaction with the ACE2 receptor (PMID: 32142651, 30626688 ). Camostat mesylate, an inhibitor of TMPRSS2, can block SARS-CoV-2 infection of lung cells (PMID: 32142651). The MW of TMPRSS2 is about 65-70 kDa. It can be cleaved into some chains with MW 54 kDa, 31 kDa and 26 kDa (PMID: 25734995, 20382709, 26018085).

实验方案

Product Specific Protocols
WB protocol for TMPRSS2 antibody 68264-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols