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验证数据展示

  • WB analysis using 68264-1-Ig

    WB analysis using 68264-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68264-1-Ig

    WB analysis using 68264-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of pig kidney using 68264-1-Ig

    WB analysis of pig kidney using 68264-1-Ig

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

TMPRSS2 Monoclonal antibody

TMPRSS2 Monoclonal Antibody for WB, ELISA
Cat No. 68264-1-Ig

产品说明书

CloneNo. 1H7E9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Pig

应用

WB, ELISA

别名

FLJ41954, PP9284, PRSS10, Serine protease 10, TMPRSS2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, pig kidney tissue, LNCaP cells, Caco-2 cells, COLO 320 cells, HT-29 cells, human testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68264-1-Ig targets TMPRSS2 in WB, ELISA applications and shows reactivity with Human, Pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Pig
免疫原 TMPRSS2 fusion protein Ag5824 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transmembrane protease, serine 2
别名 FLJ41954, PP9284, PRSS10, Serine protease 10, TMPRSS2
计算分子量 54 kDa
观测分子量50 kDa
GenBank蛋白编号BC051839
基因名称 TMPRSS2
Gene ID (NCBI) 7113
RRIDAB_2935349
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15393
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMPRSS2, also named as PRSS10, is a type II transmembrane serine protease which is highly expressed by the epithelium of the human prostate gland. TMPRSS2 may contribute to prostate tumour metastasis via the activation of PAR-2. TMPRSS2 is a Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. TMPRSS2 is a host cell factor that is critical for the spread of several clinically relevant viruses, including influenza A viruses and coronaviruses (PMID: 23468491, 30626688). SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. The initial spike protein priming by TMPRSS2 is essential for the entry and viral spread of SARS-CoV-2 through interaction with the ACE2 receptor (PMID: 32142651, 30626688 ). Camostat mesylate, an inhibitor of TMPRSS2, can block SARS-CoV-2 infection of lung cells (PMID: 32142651). The MW of TMPRSS2 is about 65-70 kDa. It can be cleaved into some chains with MW 54 kDa, 31 kDa and 26 kDa (PMID: 25734995, 20382709, 26018085).

实验方案

Product Specific Protocols
WB protocol for TMPRSS2 antibody 68264-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols