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Anti-Human TNF beta Rabbit Recombinant Antibody

TNF beta Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240393C3

Cat no : 98056-1-RR

Print datasheet

Synonyms

LTA, TNF β, Lymphotoxin-alpha, Lymphotoxin alpha, LT alpha

验证数据展示

  • FC experiment of human PBMCs using 98056-1-RR

    FC experiment of human PBMCs using 98056-1-RR

    1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human TNFB Rabbit Recombinant Antibody (98056-1-RR, Clone: 240393C3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98056-1-RR

    Affinity and Kinetic Characterization of 98056-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98056-1-RR against Human TNF Beta were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive FC (Intra) detected inAnti-CD3/CD28 treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98056-1-RR is TNF beta Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lymphotoxin alpha (TNF superfamily, member 1)
Synonyms LTA, TNF β, Lymphotoxin-alpha, Lymphotoxin alpha, LT alpha
Calculated Molecular Weight 205 aa, 22 kDa
GenBank Accession NumberBC034729
Gene Symbol TNF beta
Gene ID (NCBI) 4049
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01374
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF Beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF Beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.

实验方案

Product Specific Protocols
FC protocol for TNF beta antibody 98056-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols