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Anti-Human TNF-alpha Rabbit Recombinant Antibody, PBS Only

TNF-alpha Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240668B11

Cat No : 98064-1-PBS

Print datasheet

Synonyms

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

验证数据展示

  • FC experiment of human PBMCs using 98064-1-RR (same clone as 98064-1-PBS)

    FC experiment of human PBMCs using 98064-1-RR (same clone as 98064-1-PBS)

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human TNF-alpha Rabbit Recombinant Antibody (98064-1-RR, Clone:240668B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)Cells were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. This data was developed using the same antibody clone with 98064-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98064-1-RR (same clone as 98064-1-PBS)

    FC experiment of human PBMCs using 98064-1-RR (same clone as 98064-1-PBS)

    1x10^6 untreated(black) or PMA, Ionomycin and Brefeldin A treated(red) human PBMCs were intracellularly stained with 0.25 ug Anti-Human TNF-alpha Rabbit Recombinant Antibody (98064-1-RR, Clone:240668B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control(blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. This data was developed using the same antibody clone with 98064-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98064-1-RR

    Affinity and Kinetic Characterization of 98064-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98064-1-RR against Human TNF-alpha were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy TNF Ready-To-Use IHC Kit. TNF primary antibody included.

产品信息

The immunogen of 98064-1-PBS is TNF-alpha Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor (TNF superfamily, member 2)
Synonyms TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1
Calculated Molecular Weight26 kDa
GenBank Accession NumberNM_000594.4
Gene Symbol TNF alpha
Gene ID (NCBI) 7124
ENSEMBL Gene IDENSG00000232810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01375
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.