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验证数据展示

  • FC experiment of human PBMCs using 98064-1-RR

    FC experiment of human PBMCs using 98064-1-RR

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human TNF-alpha Rabbit Recombinant Antibody (98064-1-RR, Clone:240668B11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)Cells were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

  • FC experiment of human PBMCs using 98064-1-RR

    FC experiment of human PBMCs using 98064-1-RR

    1x10^6 untreated(black) or PMA, Ionomycin and Brefeldin A treated(red) human PBMCs were intracellularly stained with 0.25 ug Anti-Human TNF-alpha Rabbit Recombinant Antibody (98064-1-RR, Clone:240668B11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control(blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

  • Affinity and Kinetic Characterization of 98064-1-RR

    Affinity and Kinetic Characterization of 98064-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98064-1-RR against Human TNF-alpha were performed. The affinity constant is below 1 pM.

Anti-Human TNF-alpha Rabbit Recombinant Antibody

TNF-alpha Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98064-1-RR

产品说明书

CloneNo. 240668B11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inPMA, Ionomycin and Brefeldin A treated human PBMCs
Planning an IHC experiment? We recommend our IHCeasy TNF Ready-To-Use IHC Kit. TNF primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98064-1-RR targets TNF-alpha in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor (TNF superfamily, member 2)
别名 TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1
计算分子量26 kDa
GenBank蛋白编号NM_000594.4
基因名称 TNF-alpha
Gene ID (NCBI) 7124
ENSEMBL Gene IDENSG00000232810
RRIDAB_3672211
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP01375
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.