- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated TNF-alpha Monoclonal antibody
TNF-alpha Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (1)
Applications
IF-P and More (2)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
7B8A11
验证数据展示
经过测试的应用
| Positive IF-P detected in | human breast cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy TNF Ready-To-Use IHC Kit. TNF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60291-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 2 publications below |
产品信息
CL488-60291 targets TNF-alpha in WB, IF-P applications and shows reactivity with human samples.
| Tested Applications | IF-P Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | TNF-alpha fusion protein Ag11413 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | tumor necrosis factor (TNF superfamily, member 2) |
| Synonyms | TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1 |
| Calculated Molecular Weight | 233 aa, 26 kDa |
| GenBank Accession Number | BC028148 |
| Gene Symbol | TNF-alpha |
| Gene ID (NCBI) | 7124 |
| ENSEMBL Gene ID | ENSG00000232810 |
| RRID | AB_2883136 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01375 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TNF-alpha antibody CL488-60291 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Pathog SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure | ||
Biomed Pharmacother Nr2e1 ablation impairs liver glucolipid metabolism and induces inflammation, high-fat diets amplify the damage. | ||
Biomacromolecules Multifunctional Polymer Vesicles for Synergistic Antibiotic-Antioxidant Treatment of Bacterial Keratitis |