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验证数据展示

  • IF Staining of HeLa using CL594-66695

    IF Staining of HeLa using CL594-66695

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated TNFAIP3 antibody (CL594-66695, Clone: 3A11G6) at dilution of 1:200, F-actin was stained using CL488-phalloidin (green) and DNA was stained by DAPI (blue).

  • FC experiment of HeLa using CL594-66695

    FC experiment of HeLa using CL594-66695

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TNFAIP3 (CL594-66695, Clone:3A11G6) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated TNFAIP3 Monoclonal antibody

TNFAIP3 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66695

产品说明书

CloneNo. 3A11G6

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

IF/ICC, FC (Intra)

别名

OTUD7C, OTU domain-containing protein 7C, EC:2.3.2.-, A20p50, A20p37

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TNFAIP3 Ready-To-Use IHC Kit. TNFAIP3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66695-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66695 targets TNFAIP3 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, rat
免疫原 TNFAIP3 fusion protein Ag18150 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tumor necrosis factor, alpha-induced protein 3
别名 OTUD7C, OTU domain-containing protein 7C, EC:2.3.2.-, A20p50, A20p37
计算分子量 790 aa, 90 kDa
观测分子量 80 kDa
GenBank蛋白编号BC114480
基因名称 TNFAIP3
Gene ID (NCBI) 7128
RRIDAB_2883615
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP21580
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and is involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity.

实验方案

Product Specific Protocols
IF protocol for CL594 TNFAIP3 antibody CL594-66695Download protocol
FC protocol for CL594 TNFAIP3 antibody CL594-66695Download protocol
Standard Protocols
Click here to view our Standard Protocols