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CoraLite® Plus 750-conjugated TNFR2 Recombinant antibody

TNFR2 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230328B6

Cat No : CL750-83101

Print datasheet

Synonyms

TNFRSF1B, p80 TNF alpha receptor, p75TNFR, p75, Etanercept

验证数据展示

  • WB analysis using CL750-83101

    WB analysis using CL750-83101

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83101 (TNFR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inTHP-1 cells, K-562 cells, U-937 cells
Planning an IHC experiment? We recommend our IHCeasy TNFR2/TNFRSF1B Ready-To-Use IHC Kit. TNFR2/TNFRSF1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83101-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83101 targets TNFR2 in WB applications and shows reactivity with human samples.

Tested Applications WB Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 1B
Synonyms TNFRSF1B, p80 TNF alpha receptor, p75TNFR, p75, Etanercept
Calculated Molecular Weight 48 kDa
Observed Molecular Weight75 kDa, 65 kDa
GenBank Accession NumberBC052977
Gene Symbol TNFR2
Gene ID (NCBI) 7133
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20333
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 TNFR2 antibody CL750-83101Download protocol
Standard Protocols
Click here to view our Standard Protocols