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DR6 Monoclonal antibody

DR6 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C2D10

Cat no : 66754-1-Ig

Print datasheet

Synonyms

BM 018, CD358, Death receptor 6, DR6, TNFRSF21

验证数据展示

  • WB analysis of K-562 using 66754-1-Ig

    WB analysis of K-562 using 66754-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66754-1-Ig

    IF Staining of HepG2 using 66754-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DR6 antibody (66754-1-Ig, Clone: 3C2D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, THP-1 cells, Jurkat cells, Raji cells, Daudi cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66754-1-Ig targets DR6 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen DR6 fusion protein Ag14327 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 21
Synonyms BM 018, CD358, Death receptor 6, DR6, TNFRSF21
Calculated Molecular Weight 655 aa, 72 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC021572
Gene Symbol DR6
Gene ID (NCBI) 27242
RRIDAB_2882100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75509
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DR6 (also known as TNFRSF21 or CD358) is an orphan receptor of the tumor necrosis factor (TNF) receptor superfamily. It controls cell death and differentiation in a cell-autonomous manner in different cell types including neurons, oligodendrocytes, endothelial cells, and immune cells (PMID: 29459438). DR6 has been shown to activate NF-κB and JNK, and induce cell apoptosis (PMID: 9714541). DR6 interacts with TRADD, which is known to mediate signal transduction of TNF receptors. It has been reported that APP binds DR6 to trigger axon pruning and neuron death via distinct caspases, indicating that DR6 might be involved in Alzheimer's disease (PMID: 19225519).

实验方案

Product Specific Protocols
WB protocol for DR6 antibody 66754-1-IgDownload protocol
IF protocol for DR6 antibody 66754-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Discov

Knockdown of KIF15 suppresses proliferation of prostate cancer cells and induces apoptosis through PI3K/Akt signaling pathway

Authors - Hai Bi
View Article