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验证数据展示

  • WB analysis of Karpas-299 using 84241-2-RR (same clone as 84241-2-PBS)

    WB analysis of Karpas-299 using 84241-2-RR (same clone as 84241-2-PBS)

    Karpas-299 cells were subjected to SDS PAGE followed by western blot with 84241-2-RR (CD30/TNFRSF8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84241-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01137-1

    Sandwich ELISA standard curve of MP01137-1

    Sandwich ELISA standard curve of MP01137-1, Human CD30/TNFRSF8 Recombinant Matched Antibody Pair - PBS only. 84241-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02334. 84241-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-1250 pg/mL.

  • Sample test of MP01137-1

    Sample test of MP01137-1

    Serum of six individual healthy human donors was measured. The CD30/TNFRSF8 concentration of detected samples was determined to be 1,639.1 pg/mL with a range of 1,021.2-2,597.7 pg/mL.

  • Cytometric bead array standard curve of MP01137-1

    Cytometric bead array standard curve of MP01137-1

    Cytometric bead array standard curve of MP01137-1, TNFRSF8/CD30 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84241-2-PBS. Detection antibody: 84241-4-PBS. Standard: RP02334. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01137-2

    Cytometric bead array standard curve of MP01137-2

    Cytometric bead array standard curve of MP01137-2, TNFRSF8/CD30 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84241-2-PBS. Detection antibody: 84241-3-PBS. Standard: RP02334. Range: 0.625-40 ng/mL.

  • Cytometric bead array standard curve of MP01137-3

    Cytometric bead array standard curve of MP01137-3

    Cytometric bead array standard curve of MP01137-3, TNFRSF8/CD30 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84241-2-PBS. Detection antibody: 84241-1-PBS. Standard: RP02334. Range: 0.313-20 ng/mL.

  • Affinity and Kinetic Characterization of 84241-2-RR

    Affinity and Kinetic Characterization of 84241-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84241-2-RR against Human CD30/TNFRSF8 were performed. The affinity constant is 0.391 nM.

CD30/TNFRSF8 Recombinant antibody, PBS Only (Capture)

CD30/TNFRSF8 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84241-2-PBS

产品说明书

CloneNo. 241330G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

CD30, 241330G11, Ki 1 antigen, Ki 1, D1S166E

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84241-2-PBS targets CD30/TNFRSF8 as part of a matched antibody pair:

MP01137-1: 84241-2-PBS capture and 84241-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01137-2: 84241-2-PBS capture and 84241-3-PBS detection (validated in Cytometric bead array)

MP01137-3: 84241-2-PBS capture and 84241-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 8
别名 CD30, 241330G11, Ki 1 antigen, Ki 1, D1S166E
计算分子量 64 kDa
观测分子量120 kDa
GenBank蛋白编号NM_001243
基因名称 CD30
Gene ID (NCBI) 943
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP28908
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).