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CoraLite®594-conjugated TNIK Monoclonal antibody

TNIK Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E9H3

Cat No : CL594-67948

Print datasheet

Synonyms

TNIK, TRAF2 and NCK-interacting protein kinase, 1E9H3, EC:2.7.11.1, KIAA0551

验证数据展示

  • IF Staining of U2OS using CL594-67948

    IF Staining of U2OS using CL594-67948

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite®594 TNIK antibody (CL594-67948, Clone: 1E9H3 ) at dilution of 1:200.

  • FC experiment of A431 using CL594-67948

    FC experiment of A431 using CL594-67948

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human TNIK (CL594-67948, Clone:1E9H3) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inU2OS cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67948-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67948 targets TNIK in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human
Immunogen TNIK fusion protein Ag30710 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name TRAF2 and NCK interacting kinase
Synonyms TNIK, TRAF2 and NCK-interacting protein kinase, 1E9H3, EC:2.7.11.1, KIAA0551
Calculated Molecular Weight 155 kDa
GenBank Accession NumberBC055427
Gene Symbol TNIK
Gene ID (NCBI) 23043
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKE5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Traf2- and Nck-interacting kinase (TNIK) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension (PMID: 24566388). TNIK is an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462).

实验方案

Product Specific Protocols
IF protocol for CL594 TNIK antibody CL594-67948Download protocol
FC protocol for CL594 TNIK antibody CL594-67948Download protocol
Standard Protocols
Click here to view our Standard Protocols