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TNPO3 Recombinant antibody, PBS Only (Detector)

TNPO3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240728F8

Cat no : 83704-2-PBS

Print datasheet

Synonyms

transportin 3, IPO12, Importin-12, Importin 12, Imp12

验证数据展示

  • WB analysis using 83704-2-RR (same clone as 83704-2-PBS)

    WB analysis using 83704-2-RR (same clone as 83704-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83704-2-RR (TNPO3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83704-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83704-2-RR (same clone as 83704-2-PBS)

    FC experiment of HeLa using 83704-2-RR (same clone as 83704-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TNPO3 Recombinant antibody (83704-2-RR, Clone:240728F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83704-2-PBS in a different storage buffer formulation.

  • FC experiment of U-2 OS using 83704-2-RR (same clone as 83704-2-PBS)

    FC experiment of U-2 OS using 83704-2-RR (same clone as 83704-2-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug TNPO3 Recombinant antibody (83704-2-RR, Clone:240728F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83704-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00668-1

    Cytometric bead array standard curve of MP00668-1

    Cytometric bead array standard curve of MP00668-1, TNPO3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83704-1-PBS. Detection antibody: 83704-2-PBS. Standard: Ag8769 Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 83704-2-RR

    Affinity and Kinetic Characterization of 83704-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83704-2-RR against Human TNPO3 were performed. The affinity constant is below 1 pM.

产品信息

83704-2-PBS targets TNPO3 as part of a matched antibody pair:

MP00668-1: 83704-1-PBS capture and 83704-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TNPO3 fusion protein Ag8769 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transportin 3
Synonyms transportin 3, IPO12, Importin-12, Importin 12, Imp12
Calculated Molecular Weight 975 aa, 110 kDa
Observed Molecular Weight 104 kDa
GenBank Accession NumberBC009923
Gene Symbol TNPO3
Gene ID (NCBI) 23534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y5L0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transportin 3 (TNPO3) also known as IPO12, TRNSR, is a karyopherin β that works as a nuclear carrier shuttling, from the cytoplasm to the nucleus, the serine/arginine-rich proteins (PMID: 33452620). TNPO3 specifically mediates the nuclear import of splicing factor serine/arginine (SR) proteins, such as RBM4, SFRS1, and SFRS2, by recognizing phosphorylated SR domains (PMID: 11517331, 12628928). Mutations in TNPO3 are associated with Autosomal Dominant Limb-Girdle Muscular Dystrophy 2 (PMID: 23543484).