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TNPO3 Recombinant antibody

TNPO3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240728F8

Cat no : 83704-2-RR

Print datasheet

Synonyms

transportin 3, IPO12, Importin-12, Importin 12, Imp12

验证数据展示

  • WB analysis using 83704-2-RR

    WB analysis using 83704-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83704-2-RR (TNPO3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83704-2-RR

    FC experiment of HeLa using 83704-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TNPO3 Recombinant antibody (83704-2-RR, Clone:240728F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • FC experiment of U-2 OS using 83704-2-RR

    FC experiment of U-2 OS using 83704-2-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug TNPO3 Recombinant antibody (83704-2-RR, Clone:240728F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83704-2-RR

    Affinity and Kinetic Characterization of 83704-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83704-2-RR against Human TNPO3 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inJurkat cells, C2C12 cells, NIH/3T3 cells, mouse brain tissue, rat thymus tissue
Positive FC (Intra) detected inHeLa cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83704-2-RR is TNPO3 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TNPO3 fusion protein Ag8769 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transportin 3
Synonyms transportin 3, IPO12, Importin-12, Importin 12, Imp12
Calculated Molecular Weight 975 aa, 110 kDa
Observed Molecular Weight 104 kDa
GenBank Accession NumberBC009923
Gene Symbol TNPO3
Gene ID (NCBI) 23534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y5L0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transportin 3 (TNPO3) also known as IPO12, TRNSR, is a karyopherin β that works as a nuclear carrier shuttling, from the cytoplasm to the nucleus, the serine/arginine-rich proteins (PMID: 33452620). TNPO3 specifically mediates the nuclear import of splicing factor serine/arginine (SR) proteins, such as RBM4, SFRS1, and SFRS2, by recognizing phosphorylated SR domains (PMID: 11517331, 12628928). Mutations in TNPO3 are associated with Autosomal Dominant Limb-Girdle Muscular Dystrophy 2 (PMID: 23543484).

实验方案

Product Specific Protocols
WB protocol for TNPO3 antibody 83704-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols