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TOB1 Recombinant antibody, PBS Only (Detector)

TOB1 Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241043A9

Cat no : 83944-2-PBS

Print datasheet

Synonyms

Transducer of erbB 2 1, TOB, Protein Tob1, PIG49, APRO6

验证数据展示

  • IF Staining of HeLa using 83944-2-RR (same clone as 83944-2-PBS)

    IF Staining of HeLa using 83944-2-RR (same clone as 83944-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOB1 antibody (83944-2-RR, Clone: 241043A9 ) at dilution of 1:0 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83944-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00930-3

    Cytometric bead array standard curve of MP00930-3

    Cytometric bead array standard curve of MP00930-3, TOB1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83944-1-PBS. Detection antibody: 83944-2-PBS. Standard: Ag6714. Range: 0.313-20 ng/mL.

  • Affinity and Kinetic Characterization of 83944-2-RR

    Affinity and Kinetic Characterization of 83944-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83944-2-RR against Human TOB1 were performed. The affinity constant is 0.462 nM.

产品信息

83944-2-PBS targets TOB1 as part of a matched antibody pair:

MP00930-3: 83944-1-PBS capture and 83944-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen TOB1 fusion protein Ag6714 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transducer of ERBB2, 1
Synonyms Transducer of erbB 2 1, TOB, Protein Tob1, PIG49, APRO6
Calculated Molecular Weight 38 kDa
GenBank Accession NumberBC031406
Gene Symbol TOB1
Gene ID (NCBI) 10140
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50616
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.