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TOB1 Recombinant antibody

TOB1 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241043A9

Cat no : 83944-2-RR

Print datasheet

Synonyms

Transducer of erbB 2 1, TOB, Protein Tob1, PIG49, APRO6

验证数据展示

  • IF Staining of HeLa using 83944-2-RR

    IF Staining of HeLa using 83944-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOB1 antibody (83944-2-RR, Clone: 241043A9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83944-2-RR

    Affinity and Kinetic Characterization of 83944-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83944-2-RR against Human TOB1 were performed. The affinity constant is 0.462 nM.

经过测试的应用

Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83944-2-RR targets TOB1 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen TOB1 fusion protein Ag6714 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transducer of ERBB2, 1
Synonyms Transducer of erbB 2 1, TOB, Protein Tob1, PIG49, APRO6
Calculated Molecular Weight 38 kDa
GenBank Accession NumberBC031406
Gene Symbol TOB1
Gene ID (NCBI) 10140
RRIDAB_3671524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50616
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.

实验方案

Product Specific Protocols
IF protocol for TOB1 antibody 83944-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols