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TOE1 Polyclonal antibody

TOE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16203-1-AP

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Synonyms

验证数据展示

  • WB analysis using 16203-1-AP

    WB analysis using 16203-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16203-1-AP (TOE1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16203-1-AP

    IF Staining of HeLa using 16203-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOE1 antibody (16203-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IHC staining of human breast cancer using 16203-1-AP

    IHC staining of human breast cancer using 16203-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16203-1-AP (TOE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse thymus tissue, HeLa cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

16203-1-AP targets TOE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen TOE1 fusion protein Ag9172 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name target of EGR1, member 1 (nuclear)
Synonyms
Calculated Molecular Weight 510 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC009364
Gene Symbol TOE1
Gene ID (NCBI) 114034
RRIDAB_2207278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96GM8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.

实验方案

Product Specific Protocols
WB protocol for TOE1 antibody 16203-1-APDownload protocol
IHC protocol for TOE1 antibody 16203-1-APDownload protocol
IF protocol for TOE1 antibody 16203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Theriogenology

Knockdown of Toe1 causes developmental arrest during the morula-to-blastocyst transition in mice

Authors - Hongcheng Wang
View Article