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验证数据展示

  • IF Staining of HeLa using CL488-16203

    IF Staining of HeLa using CL488-16203

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TOE1 antibody (CL488-16203) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-16203

    FC experiment of HeLa using CL488-16203

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated TOE1 Polyclonal antibody (CL488-16203)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TOE1 Polyclonal antibody

TOE1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-16203

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

Target of EGR1 protein 1, TOE1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 16203-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16203 targets TOE1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen TOE1 fusion protein Ag9172 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name target of EGR1, member 1 (nuclear)
Synonyms Target of EGR1 protein 1, TOE1
Calculated Molecular Weight 510 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC009364
Gene Symbol TOE1
Gene ID (NCBI) 114034
RRIDAB_3672646
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96GM8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TOE1 antibody CL488-16203Download protocol
FC protocol for CL Plus 488 TOE1 antibody CL488-16203Download protocol
Standard Protocols
Click here to view our Standard Protocols