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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67829-1-Ig (same clone as 67829-1-PBS)

    WB analysis using 67829-1-Ig (same clone as 67829-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67829-1-Ig (TOE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67829-1-Ig (same clone as 67829-1-PBS)

    IHC staining of human colon cancer using 67829-1-Ig (same clone as 67829-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67829-1-Ig (same clone as 67829-1-PBS)

    IHC staining of human colon cancer using 67829-1-Ig (same clone as 67829-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67829-1-Ig (same clone as 67829-1-PBS)

    IF Staining of HepG2 using 67829-1-Ig (same clone as 67829-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOE1 antibody (67829-1-Ig, Clone: 2C10H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

TOE1 Monoclonal antibody, PBS Only

TOE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67829-1-PBS

Print datasheet

CloneNo. 2C10H7

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FLJ13949, Target of EGR1 protein 1, TOE1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

67829-1-PBS targets TOE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen TOE1 fusion protein Ag28982 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name target of EGR1, member 1 (nuclear)
Synonyms FLJ13949, Target of EGR1 protein 1, TOE1
Calculated Molecular Weight 510 aa, 57 kDa
Observed Molecular Weight57 kDa
GenBank Accession NumberBC009364
Gene Symbol TOE1
Gene ID (NCBI) 114034
RRIDAB_2918591
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96GM8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.