• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84711-5-RR (same clone as 84711-5-PBS)

    WB analysis using 84711-5-RR (same clone as 84711-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84711-5-RR (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84711-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84711-5-RR (same clone as 84711-5-PBS)

    IF Staining of HepG2 using 84711-5-RR (same clone as 84711-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TOLLIP antibody (84711-5-RR, Clone: 242098E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84711-5-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84711-5-RR (same clone as 84711-5-PBS)

    FC experiment of U2OS using 84711-5-RR (same clone as 84711-5-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug TOLLIP Recombinant antibody (84711-5-RR, Clone:242098E3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84711-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84711-5-RR

    Affinity and Kinetic Characterization of 84711-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84711-5-RR against Human TOLLIP were performed. The affinity constant is 0.309 nM.

TOLLIP Recombinant antibody, PBS Only

TOLLIP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84711-5-PBS

产品说明书

CloneNo. 242098E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 242098E3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy TOLLIP Ready-To-Use IHC Kit. TOLLIP primary antibody included.

产品信息

84711-5-PBS targets TOLLIP in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TOLLIP fusion protein Ag1864 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 toll interacting protein
别名 Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 242098E3
计算分子量 30 kDa
观测分子量30 kDa
GenBank蛋白编号BC012057
基因名称 TOLLIP
Gene ID (NCBI) 54472
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H0E2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).