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TOM20 Recombinant antibody, PBS Only

TOM20 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, chicken, zebrafish

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D22

Cat no : 80501-1-PBS

Print datasheet

Synonyms

TOMM20, TOMM 20, tom 20, MOM19, MAS20

验证数据展示

  • WB analysis using 80501-1-RR (same clone as 80501-1-PBS)

    WB analysis using 80501-1-RR (same clone as 80501-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80501-1-RR (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80501-1-RR (same clone as 80501-1-PBS)

    IF Staining of HepG2 using 80501-1-RR (same clone as 80501-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (80501-1-RR, Clone: 1D22 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 80501-1-RR (same clone as 80501-1-PBS)

    IHC staining of human liver using 80501-1-RR (same clone as 80501-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80501-1-RR (TOM20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TOMM20 antibody included
  • Affinity and Kinetic Characterization of 80501-1-RR

    Affinity and Kinetic Characterization of 80501-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80501-1-RR against Human TOM20 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy TOMM20 Ready-To-Use IHC Kit. TOMM20 primary antibody included.

产品信息

80501-1-PBS targets TOM20 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, chicken, zebrafish
Immunogen TOM20 fusion protein Ag2378 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name translocase of outer mitochondrial membrane 20 homolog (yeast)
Synonyms TOMM20, TOMM 20, tom 20, MOM19, MAS20
Calculated Molecular Weight 145 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC000882
Gene Symbol TOM20
Gene ID (NCBI) 9804
RRIDAB_2918897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15388
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.