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TOM20 Recombinant antibody

TOM20 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, chicken, zebrafish

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D22

Cat no : 80501-1-RR

Print datasheet

Synonyms

TOMM20, TOMM 20, tom 20, MOM19, MAS20

验证数据展示

  • WB analysis using 80501-1-RR

    WB analysis using 80501-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80501-1-RR (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 80501-1-RR

    IF Staining of HepG2 using 80501-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (80501-1-RR, Clone: 1D22 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human liver using 80501-1-RR

    IHC staining of human liver using 80501-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80501-1-RR (TOM20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 80501-1-RR

    Affinity and Kinetic Characterization of 80501-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80501-1-RR against Human TOM20 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, zebrafish tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80501-1-RR targets TOM20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, chicken, zebrafish
Immunogen TOM20 fusion protein Ag2378 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name translocase of outer mitochondrial membrane 20 homolog (yeast)
Synonyms TOMM20, TOMM 20, tom 20, MOM19, MAS20
Calculated Molecular Weight 145 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC000882
Gene Symbol TOM20
Gene ID (NCBI) 9804
RRIDAB_2918897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15388
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.

实验方案

Product Specific Protocols
WB protocol for TOM20 antibody 80501-1-RRDownload protocol
IHC protocol for TOM20 antibody 80501-1-RRDownload protocol
IF protocol for TOM20 antibody 80501-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols