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TOM70 Monoclonal antibody

TOM70 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66593-1-Ig

产品说明书

CloneNo. 1H8A1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat, Pig and More (2)

应用

WB, IHC, IF/ICC, ELISA and More (1)

KIAA0719, KIAA0719, TOM70, TOM70, TOMM70A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66593-1-Ig targets TOM70 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 Human, Mouse, Rat, Pig
文献引用反应性human, mouse
免疫原 TOM70 fusion protein Ag6027 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
别名 KIAA0719, KIAA0719, TOM70, TOM70, TOMM70A
计算分子量 67 kDa
观测分子量 70 kDa
GenBank蛋白编号BC052994
基因名称 TOM70
Gene ID (NCBI) 9868
RRIDAB_2881953
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO94826
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOM70 is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.

实验方案

Product Specific Protocols
WB protocol for TOM70 antibody 66593-1-IgDownload protocol
IHC protocol for TOM70 antibody 66593-1-IgDownload protocol
IF protocol for TOM70 antibody 66593-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Science

Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.

Authors - Qiang Wang
humanWB

Mol Cell

Dynamic Regulation of Mitochondrial Import by the Ubiquitin System.

Authors - Lilian Phu
mouseWB

Physiol Rep

TOM complex-independent transport pathway of myoglobin into mitochondria in C2C12 myotubes

Authors - Rikuhide Koma
mouseIF

Asian J Androl

Caseinolytic mitochondrial matrix peptidase X is essential for homologous chromosome synapsis and recombination during meiosis of male mouse germ cells

Authors - Hai-Wei Feng
  • KO Validated
humanWB

Mol Cell

Reactive oxygen species control protein degradation at the mitochondrial import gate

Authors - Rachael McMinimy
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