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验证数据展示

  • WB analysis using 66593-1-Ig (same clone as 66593-1-PBS)

    WB analysis using 66593-1-Ig (same clone as 66593-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66593-1-Ig (TOM70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66593-1-Ig (same clone as 66593-1-PBS)

    IF Staining of HeLa using 66593-1-Ig (same clone as 66593-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66593-1-Ig (TOM70 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66593-1-Ig (same clone as 66593-1-PBS)

    IHC staining of mouse brain using 66593-1-Ig (same clone as 66593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66593-1-Ig (same clone as 66593-1-PBS)

    IHC staining of mouse brain using 66593-1-Ig (same clone as 66593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TOM70 Monoclonal antibody, PBS Only

TOM70 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66593-1-PBS

产品说明书

CloneNo. 1H8A1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

TOMM70A, Translocase of outer mitochondrial membrane protein 70, TOMM70, TOMM, KIAA0719

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66593-1-PBS targets TOM70 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 TOM70 fusion protein Ag6027 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
别名 TOMM70A, Translocase of outer mitochondrial membrane protein 70, TOMM70, TOMM, KIAA0719
计算分子量 67 kDa
观测分子量 70 kDa
GenBank蛋白编号BC052994
基因名称 TOM70
Gene ID (NCBI) 9868
RRIDAB_2881953
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO94826
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TOM70 is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.