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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TOP2A Polyclonal antibody

TOP2A Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24641

Print datasheet

Synonyms

DNA topoisomerase 2 alpha, TOP2, TOP2A, TP2A

验证数据展示

  • IF Staining of A431 using CL488-24641

    IF Staining of A431 using CL488-24641

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 TOP2A antibody (CL488-24641) at dilution of 1:400.

经过测试的应用

Positive IF/ICC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 24641-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24641 targets TOP2A in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen TOP2A fusion protein Ag19551 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name topoisomerase (DNA) II alpha 170kDa
Synonyms DNA topoisomerase 2 alpha, TOP2, TOP2A, TP2A
Calculated Molecular Weight 1531 aa, 174 kDa
Observed Molecular Weight 174 kDa
GenBank Accession NumberBC140791
Gene Symbol TOP2A
Gene ID (NCBI) 7153
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11388
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TOP2A antibody CL488-24641Download protocol
Standard Protocols
Click here to view our Standard Protocols