验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 24641-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-24641 targets TOP2A in IF/ICC applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | TOP2A fusion protein Ag19551 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | topoisomerase (DNA) II alpha 170kDa |
| 别名 | DNA topoisomerase 2 alpha, TOP2, TOP2A, TP2A |
| 计算分子量 | 1531 aa, 174 kDa |
| 观测分子量 | 174 kDa |
| GenBank蛋白编号 | BC140791 |
| 基因名称 | TOP2A |
| Gene ID (NCBI) | 7153 |
| RRID | AB_3084084 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P11388 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TOP2A antibody CL488-24641 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |