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验证数据展示

  • FC experiment of A549 using 84304-2-RR (same clone as 84304-2-PBS)

    FC experiment of A549 using 84304-2-RR (same clone as 84304-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Tox2 Recombinant Antibody (84304-2-RR, Clone:241645A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84304-2-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 84304-2-RR (same clone as 84304-2-PBS)

    IF Staining of A549 using 84304-2-RR (same clone as 84304-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TOX2 antibody (84304-2-RR, Clone: 241645A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84304-2-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 84304-2-RR (same clone as 84304-2-PBS)

    IF Staining of A549 using 84304-2-RR (same clone as 84304-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TOX2 antibody (84304-2-RR, Clone: 241645A11 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 84304-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01188-1

    Cytometric bead array standard curve of MP01188-1

    Cytometric bead array standard curve of MP01188-1, TOX2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84304-2-PBS. Detection antibody: 84304-1-PBS. Standard: Ag14772. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP01188-2

    Cytometric bead array standard curve of MP01188-2

    Cytometric bead array standard curve of MP01188-2, TOX2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84304-2-PBS. Detection antibody: 84304-4-PBS. Standard: Ag14772. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP01188-3

    Cytometric bead array standard curve of MP01188-3

    Cytometric bead array standard curve of MP01188-3, TOX2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84304-2-PBS. Detection antibody: 84304-3-PBS. Standard: Ag14772. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 84304-2-RR

    Affinity and Kinetic Characterization of 84304-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84304-2-RR against Human TOX2 were performed. The affinity constant is 0.240 nM.

TOX2 Recombinant antibody, PBS Only (Capture)

TOX2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84304-2-PBS

产品说明书

CloneNo. 241645A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

GCX-1, GCX1, C20orf100, 241645A11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84304-2-PBS targets TOX2 as part of a matched antibody pair:

MP01188-1: 84304-2-PBS capture and 84304-1-PBS detection (validated in Cytometric bead array)

MP01188-2: 84304-2-PBS capture and 84304-4-PBS detection (validated in Cytometric bead array)

MP01188-3: 84304-2-PBS capture and 84304-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 TOX2 fusion protein Ag14772 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TOX high mobility group box family member 2
别名 GCX-1, GCX1, C20orf100, 241645A11
计算分子量 488 aa, 52 kDa
GenBank蛋白编号BC007636
基因名称 TOX2
Gene ID (NCBI) 84969
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96NM4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).