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验证数据展示

  • FC experiment of A549 using 84304-2-RR

    FC experiment of A549 using 84304-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Tox2 Recombinant Antibody (84304-2-RR, Clone:241645A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of A549 using 84304-2-RR

    IF Staining of A549 using 84304-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TOX2 antibody (84304-2-RR, Clone: 241645A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A549 using 84304-2-RR

    IF Staining of A549 using 84304-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TOX2 antibody (84304-2-RR, Clone: 241645A11 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

  • Affinity and Kinetic Characterization of 84304-2-RR

    Affinity and Kinetic Characterization of 84304-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84304-2-RR against Human TOX2 were performed. The affinity constant is 0.240 nM.

TOX2 Recombinant antibody

TOX2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84304-2-RR

产品说明书

CloneNo. 241645A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

GCX-1, GCX1, C20orf100, 241645A11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84304-2-RR targets TOX2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 TOX2 fusion protein Ag14772 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TOX high mobility group box family member 2
别名 GCX-1, GCX1, C20orf100, 241645A11
计算分子量 488 aa, 52 kDa
GenBank蛋白编号BC007636
基因名称 TOX2
Gene ID (NCBI) 84969
RRIDAB_3671844
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96NM4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).

实验方案

Product Specific Protocols
IF protocol for TOX2 antibody 84304-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols