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  • Featured Product
  • KD/KO Validated

TPC1 Polyclonal antibody, PBS Only

TPC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23758-1-PBS

Print datasheet

Synonyms

TPCN1, Voltage-dependent calcium channel protein TPC1, two pore segment channel 1, Two pore channel protein 1, KIAA1169

验证数据展示

  • WB analysis using 23758-1-AP (same clone as 23758-1-PBS)

    WB analysis using 23758-1-AP (same clone as 23758-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23758-1-AP (TPC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23758-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 23758-1-AP (same clone as 23758-1-PBS)

    IHC staining of mouse heart using 23758-1-AP (same clone as 23758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23758-1-AP (TPCN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23758-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 23758-1-AP (same clone as 23758-1-PBS)

    IF Staining of HepG2 using 23758-1-AP (same clone as 23758-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TPC1 antibody (23758-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 23758-1-PBS in a different storage buffer formulation.

产品信息

23758-1-PBS targets TPC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TPC1 fusion protein Ag20607 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name two pore segment channel 1
Synonyms TPCN1, Voltage-dependent calcium channel protein TPC1, two pore segment channel 1, Two pore channel protein 1, KIAA1169
Calculated Molecular Weight 816 aa, 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC136795
Gene Symbol TPCN1
Gene ID (NCBI) 53373
RRIDAB_2879317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9ULQ1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Two-pore channels (TPCs) are ancient members of the voltage-gated ion channel superfamily, are thought to be non-selective cation channels permeable to Ca2+. They mediate their physiological effects through releasing Ca2+ from acidic organelles in response to cues such as the second messenger, NAADP (nicotinic acid adenine dinucleotide phosphate). Genetic knockout and pharmacological inhibition experiments demonstrate that the two-pore channels, TPC1 and TPC2, are required for infection by Filoviruses Ebola and Marburg in mice.(PMID: 29746898; PMID 25722412) Two pore segment channel 1 (TPC1) is a human protein encoded by the TPCN1 gene.