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  • Featured Product
  • KD/KO Validated

TPM4 Monoclonal antibody, PBS Only

TPM4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, pig, rat, mouse, human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E12D12

Cat no : 67244-1-PBS

Print datasheet

Synonyms

Tropomyosin-4, Tropomyosin alpha-4 chain, Tropomyosin alpha 4 chain, tropomyosin 4, TM30p1

验证数据展示

  • WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 67244-1-Ig (same clone as 67244-1-PBS)

    WB analysis of A549 using 67244-1-Ig (same clone as 67244-1-PBS)

    WB result of TPM4 antibody (67244-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM4 transfected A549 cells. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 67244-1-Ig (same clone as 67244-1-PBS)

    IHC staining of mouse heart using 67244-1-Ig (same clone as 67244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPM4 antibody included
  • IHC staining of rat heart using 67244-1-Ig (same clone as 67244-1-PBS)

    IHC staining of rat heart using 67244-1-Ig (same clone as 67244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPM4 antibody included
  • IF Staining of MCF-7 using 67244-1-Ig (same clone as 67244-1-PBS)

    IF Staining of MCF-7 using 67244-1-Ig (same clone as 67244-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TPM4 antibody (67244-1-Ig, Clone: 2E12D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TPM4 Ready-To-Use IHC Kit. TPM4 primary antibody included.

产品信息

67244-1-PBS targets TPM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity rabbit, pig, rat, mouse, human
Immunogen TPM4 fusion protein Ag6947 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tropomyosin 4
Synonyms Tropomyosin-4, Tropomyosin alpha-4 chain, Tropomyosin alpha 4 chain, tropomyosin 4, TM30p1
Calculated Molecular Weight 248 aa, 29 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberBC037576
Gene Symbol TPM4
Gene ID (NCBI) 7171
ENSEMBL Gene IDENSG00000167460
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP67936
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPM4 is a member of tropomyosins (TPMs), a multi-gene family of actin-binding proteins present in all eukaryotic cells. In muscle, TPMs are responsible for mediating contraction via regulation of the actin-myosin interaction. As to non-muscle cells, its proposed role is to stabilize the actin filaments by modulating the interaction with proteins that are responsible for the regulation of actin dynamics. The form of TPM4 in muscle has a higher molecular weight than the form found in non-muscle cells.