• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    WB analysis using 67244-1-Ig (same clone as 67244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 67244-1-Ig (same clone as 67244-1-PBS)

    WB analysis of A549 using 67244-1-Ig (same clone as 67244-1-PBS)

    WB result of TPM4 antibody (67244-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM4 transfected A549 cells. This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 67244-1-Ig (same clone as 67244-1-PBS)

    IHC staining of mouse heart using 67244-1-Ig (same clone as 67244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPM4 antibody included
  • IHC staining of rat heart using 67244-1-Ig (same clone as 67244-1-PBS)

    IHC staining of rat heart using 67244-1-Ig (same clone as 67244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPM4 antibody included
  • IF Staining of MCF-7 using 67244-1-Ig (same clone as 67244-1-PBS)

    IF Staining of MCF-7 using 67244-1-Ig (same clone as 67244-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TPM4 antibody (67244-1-Ig, Clone: 2E12D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67244-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TPM4 Monoclonal antibody, PBS Only

TPM4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67244-1-PBS

产品说明书

CloneNo. 2E12D12

宿主/亚型

Mouse / IgG1

种属反应性

rabbit, pig, rat, mouse, human

应用

WB, IHC, IF/ICC, ELISA

别名

Tropomyosin-4, Tropomyosin alpha-4 chain, Tropomyosin alpha 4 chain, tropomyosin 4, TM30p1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy TPM4 Ready-To-Use IHC Kit. TPM4 primary antibody included.

产品信息

67244-1-PBS targets TPM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 rabbit, pig, rat, mouse, human
免疫原 TPM4 fusion protein Ag6947 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tropomyosin 4
别名 Tropomyosin-4, Tropomyosin alpha-4 chain, Tropomyosin alpha 4 chain, tropomyosin 4, TM30p1
计算分子量 248 aa, 29 kDa
观测分子量 32-35 kDa
GenBank蛋白编号BC037576
基因名称 TPM4
Gene ID (NCBI) 7171
ENSEMBL Gene IDENSG00000167460
RRIDAB_2882523
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP67936
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPM4 is a member of tropomyosins (TPMs), a multi-gene family of actin-binding proteins present in all eukaryotic cells. In muscle, TPMs are responsible for mediating contraction via regulation of the actin-myosin interaction. As to non-muscle cells, its proposed role is to stabilize the actin filaments by modulating the interaction with proteins that are responsible for the regulation of actin dynamics. The form of TPM4 in muscle has a higher molecular weight than the form found in non-muscle cells.