验证数据展示
经过测试的应用
| Positive WB detected in | rat skeletal muscle tissue, A549 cells, HeLa cells, HepG2 cells, mouse skeletal muscle tissue, rabbit skeletal muscle tissue, rat heart tissue, mouse heart tissue, rabbit heart tissue, HT-29 cells, human heart tissue, pig heart tissue |
| Positive IHC detected in | mouse heart tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy TPM4 Ready-To-Use IHC Kit. TPM4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
67244-1-Ig targets TPM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 文献引用反应性 | human, mouse |
| 免疫原 | TPM4 fusion protein Ag6947 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tropomyosin 4 |
| 别名 | Tropomyosin-4, Tropomyosin alpha-4 chain, Tropomyosin alpha 4 chain, tropomyosin 4, TM30p1 |
| 计算分子量 | 248 aa, 29 kDa |
| 观测分子量 | 32-35 kDa |
| GenBank蛋白编号 | BC037576 |
| 基因名称 | TPM4 |
| Gene ID (NCBI) | 7171 |
| ENSEMBL Gene ID | ENSG00000167460 |
| RRID | AB_2882523 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P67936 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TPM4 is a member of tropomyosins (TPMs), a multi-gene family of actin-binding proteins present in all eukaryotic cells. In muscle, TPMs are responsible for mediating contraction via regulation of the actin-myosin interaction. As to non-muscle cells, its proposed role is to stabilize the actin filaments by modulating the interaction with proteins that are responsible for the regulation of actin dynamics. The form of TPM4 in muscle has a higher molecular weight than the form found in non-muscle cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TPM4 antibody 67244-1-Ig | Download protocol |
| IHC protocol for TPM4 antibody 67244-1-Ig | Download protocol |
| IF protocol for TPM4 antibody 67244-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Rheumatology (Oxford) Association of Anti-TPM4 autoantibodies with vasculopathic cutaneous manifestations in juvenile dermatomyositis | ||
PLoS One Proteomic changes in the hippocampus of large mammals after total-body low dose radiation | ||
Cell Discov TPM4 condensates glycolytic enzymes and facilitates actin reorganization under hyperosmotic stress |