验证数据展示
经过测试的应用
| Positive WB detected in | rat skeletal muscle tissue, mouse skeletal muscle tissue |
Planning an IHC experiment? We recommend our IHCeasy TPM4 Ready-To-Use IHC Kit. TPM4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67244-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67244 targets TPM4 in WB applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Applications | WB Application Description |
| Tested Reactivity | Human, mouse, rat, pig |
| Immunogen | TPM4 fusion protein Ag6947 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | tropomyosin 4 |
| Synonyms | TM30p1, TPM4, tropomyosin 4, Tropomyosin alpha 4 chain |
| Calculated Molecular Weight | 248 aa, 29 kDa |
| Observed Molecular Weight | 32-35 kDa |
| GenBank Accession Number | BC037576 |
| Gene Symbol | TPM4 |
| Gene ID (NCBI) | 7171 |
| ENSEMBL Gene ID | ENSG00000167460 |
| RRID | AB_3084330 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P67936 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TPM4 is a member of tropomyosins (TPMs), a multi-gene family of actin-binding proteins present in all eukaryotic cells. In muscle, TPMs are responsible for mediating contraction via regulation of the actin-myosin interaction. As to non-muscle cells, its proposed role is to stabilize the actin filaments by modulating the interaction with proteins that are responsible for the regulation of actin dynamics. The form of TPM4 in muscle has a higher molecular weight than the form found in non-muscle cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 TPM4 antibody CL488-67244 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |