验证数据展示
经过测试的应用
| Positive WB detected in | rat skeletal muscle tissue, mouse skeletal muscle tissue |
Planning an IHC experiment? We recommend our IHCeasy TPM4 Ready-To-Use IHC Kit. TPM4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67244-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67244 targets TPM4 in WB applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human, mouse, rat, pig |
| 免疫原 | TPM4 fusion protein Ag6947 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tropomyosin 4 |
| 别名 | TM30p1, TPM4, tropomyosin 4, Tropomyosin alpha 4 chain |
| 计算分子量 | 248 aa, 29 kDa |
| 观测分子量 | 32-35 kDa |
| GenBank蛋白编号 | BC037576 |
| 基因名称 | TPM4 |
| Gene ID (NCBI) | 7171 |
| ENSEMBL Gene ID | ENSG00000167460 |
| RRID | AB_3084330 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P67936 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TPM4 is a member of tropomyosins (TPMs), a multi-gene family of actin-binding proteins present in all eukaryotic cells. In muscle, TPMs are responsible for mediating contraction via regulation of the actin-myosin interaction. As to non-muscle cells, its proposed role is to stabilize the actin filaments by modulating the interaction with proteins that are responsible for the regulation of actin dynamics. The form of TPM4 in muscle has a higher molecular weight than the form found in non-muscle cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 TPM4 antibody CL488-67244 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |