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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated TPPII Monoclonal antibody

TPPII Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5H6F11

Cat no : CL594-66017

Print datasheet

Synonyms

FLJ40359, TPP 2, TPP II, TPP2, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II

验证数据展示

  • IF Staining of HeLa using CL594-66017

    IF Staining of HeLa using CL594-66017

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66017 (TPPII antibody) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66017-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-66017 is TPPII Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen TPPII fusion protein Ag5296 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tripeptidyl peptidase II
Synonyms FLJ40359, TPP 2, TPP II, TPP2, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II
Calculated Molecular Weight 1249 aa, 138 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC039905
Gene Symbol TPPII
Gene ID (NCBI) 7174
RRIDAB_2883479
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29144
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

实验方案

Product Specific Protocols
IF protocol for CL594 TPPII antibody CL594-66017Download protocol
Standard Protocols
Click here to view our Standard Protocols