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验证数据展示

  • WB analysis using 68048-1-Ig

    WB analysis using 68048-1-Ig

    Brain tissues were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human lung using 68048-1-Ig

    IHC staining of human lung using 68048-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPPP antibody included
  • IHC staining of human lung using 68048-1-Ig

    IHC staining of human lung using 68048-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPPP antibody included
  • WB analysis of SH-SY5Y using 68048-1-Ig

    WB analysis of SH-SY5Y using 68048-1-Ig

    WB result of TPPP antibody (68048-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPPP transfected SH-SY5Y cells.

  • IF Staining of Neuro-2a using 68048-1-Ig

    IF Staining of Neuro-2a using 68048-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using TPPP antibody (68048-1-Ig, Clone: 1E5F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Featured Product
  • KD/KO Validated

TPPP Monoclonal antibody

TPPP Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68048-1-Ig
CloneNo. 1E5F5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

p25 alpha, p24, EC:3.6.5.-, 25 kDa brain-specific protein, 25 kDa brain specific protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue, SH-SY5Y cells, rabbit brain, rat brain, mouse brain, chicken brain
Positive IHC detected inhuman lung tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy TPPP Ready-To-Use IHC Kit. TPPP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68048-1-Ig targets TPPP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit, chicken
Immunogen TPPP fusion protein Ag19046 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tubulin polymerization promoting protein
Synonyms p25 alpha, p24, EC:3.6.5.-, 25 kDa brain-specific protein, 25 kDa brain specific protein
Calculated Molecular Weight 219 aa, 24 kDa
Observed Molecular Weight24-29 kDa
GenBank Accession NumberBC131506
Gene Symbol TPPP
Gene ID (NCBI) 11076
RRIDAB_2918790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94811
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPPP, also named as TPPP1, P24 and P25, belongs to the TPPP family. TPPP promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.

实验方案

Product Specific Protocols
WB protocol for TPPP antibody 68048-1-IgDownload protocol
IHC protocol for TPPP antibody 68048-1-IgDownload protocol
IF protocol for TPPP antibody 68048-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols