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  • Featured Product
  • KD/KO Validated

TPPP Monoclonal antibody, PBS Only

TPPP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E5F5

Cat no : 68048-1-PBS

Print datasheet

Synonyms

p25 alpha, p24, EC:3.6.5.-, 25 kDa brain-specific protein, 25 kDa brain specific protein

验证数据展示

  • WB analysis using 68048-1-Ig (same clone as 68048-1-PBS)

    WB analysis using 68048-1-Ig (same clone as 68048-1-PBS)

    Brain tissues were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 68048-1-Ig (same clone as 68048-1-PBS)

    IHC staining of human lung using 68048-1-Ig (same clone as 68048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPPP antibody included
  • IHC staining of human lung using 68048-1-Ig (same clone as 68048-1-PBS)

    IHC staining of human lung using 68048-1-Ig (same clone as 68048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPPP antibody included
  • WB analysis of SH-SY5Y using 68048-1-Ig (same clone as 68048-1-PBS)

    WB analysis of SH-SY5Y using 68048-1-Ig (same clone as 68048-1-PBS)

    WB result of TPPP antibody (68048-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPPP transfected SH-SY5Y cells. This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 68048-1-Ig (same clone as 68048-1-PBS)

    IF Staining of Neuro-2a using 68048-1-Ig (same clone as 68048-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using TPPP antibody (68048-1-Ig, Clone: 1E5F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TPPP Ready-To-Use IHC Kit. TPPP primary antibody included.

产品信息

68048-1-PBS targets TPPP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit, chicken
Immunogen TPPP fusion protein Ag19046 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tubulin polymerization promoting protein
Synonyms p25 alpha, p24, EC:3.6.5.-, 25 kDa brain-specific protein, 25 kDa brain specific protein
Calculated Molecular Weight 219 aa, 24 kDa
Observed Molecular Weight24-29 kDa
GenBank Accession NumberBC131506
Gene Symbol TPPP
Gene ID (NCBI) 11076
RRIDAB_2918790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94811
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPPP, also named as TPPP1, P24 and P25, belongs to the TPPP family. TPPP promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.