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验证数据展示

  • WB analysis using 83177-5-RR

    WB analysis using 83177-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83177-5-RR (TPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 83177-5-RR

    IHC staining of mouse testis using 83177-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83177-5-RR (TPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 83177-5-RR

    IF Staining of HeLa using 83177-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TPR antibody (83177-5-RR, Clone: 230413A2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

TPR Recombinant antibody

TPR Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83177-5-RR

产品说明书

CloneNo. 230413A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Nucleoprotein TPR, 230413A2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83177-5-RR targets TPR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 translocated promoter region (to activated MET oncogene)
别名 Nucleoprotein TPR, 230413A2
计算分子量 267 kDa
观测分子量~270 kDa
GenBank蛋白编号NM_003292
基因名称 TPR
Gene ID (NCBI) 7175
RRIDAB_3670870
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP12270
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleoporin TPR was originally identified as the oncogenic activator of the met, raf, and trk protooncogenes. TPR is a large 270 kDa protein with a bipartite structure consisting of a ~1600-residue a-helical coiled-coil N-terminal domain and a highly acidic non-coiled ~800 amino acid carboxy terminus (PMID: 22253824). TPR is localized exclusively to intranuclear filaments associated with the nucleoplasmic side of the NPC, by directly binding to Nup153 (PMID: 12802065).

实验方案

Product Specific Protocols
WB protocol for TPR antibody 83177-5-RRDownload protocol
IHC protocol for TPR antibody 83177-5-RRDownload protocol
IF protocol for TPR antibody 83177-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols