CoraLite® Plus 488-conjugated TPR Recombinant antibody

TPR Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-83177-5
CloneNo. 230413A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -


经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 83177-5-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83177-5 targets TPR in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name translocated promoter region (to activated MET oncogene)
Synonyms
Calculated Molecular Weight 267 kDa
Observed Molecular Weight~270 kDa
GenBank Accession NumberNM_003292
Gene Symbol TPR
Gene ID (NCBI) 7175
RRIDAB_3673216
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12270
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleoporin TPR was originally identified as the oncogenic activator of the met, raf, and trk protooncogenes. TPR is a large 270 kDa protein with a bipartite structure consisting of a ~1600-residue a-helical coiled-coil N-terminal domain and a highly acidic non-coiled ~800 amino acid carboxy terminus (PMID: 22253824). TPR is localized exclusively to intranuclear filaments associated with the nucleoplasmic side of the NPC, by directly binding to Nup153 (PMID: 12802065).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TPR antibody CL488-83177-5Download protocol
Standard Protocols
Click here to view our Standard Protocols
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