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TPTE2 Polyclonal antibody

TPTE2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 25120-1-AP

Print datasheet

Synonyms

Lipid phosphatase TPIP, TPIP, TPTE2

验证数据展示

  • WB analysis of L02 using 25120-1-AP

    WB analysis of L02 using 25120-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 25120-1-AP (TPTE2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IHC staining of human liver cancer using 25120-1-AP

    IHC staining of human liver cancer using 25120-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25120-1-AP (TPTE2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 25120-1-AP

    IHC staining of human liver cancer using 25120-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25120-1-AP (TPTE2 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of L02 using 25120-1-AP

    IF Staining of L02 using 25120-1-AP

    Immunofluorescent analysis of L02 cells using 25120-1-AP (TPTE2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25120-1-AP is TPTE2 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen TPTE2 fusion protein Ag19169 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane phosphoinositide 3-phosphatase and tensin homolog 2
Synonyms Lipid phosphatase TPIP, TPIP, TPTE2
Calculated Molecular Weight 522 aa, 61 kDa
Observed Molecular Weight 38-60 kDa
GenBank Accession NumberBC128146
Gene Symbol TPTE2
Gene ID (NCBI) 93492
RRIDAB_2879908
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6XPS3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TPTE2 antibody 25120-1-APDownload protocol
IHC protocol for TPTE2 antibody 25120-1-APDownload protocol
IF protocol for TPTE2 antibody 25120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols