验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, K-562 cells, HeLa cells, SMMC-7721 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11741-1-AP targets TPX2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TPX2 fusion protein Ag2334 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TPX2, microtubule-associated, homolog (Xenopus laevis) |
| 别名 | DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2 |
| 计算分子量 | 747 aa, 86 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC020207 |
| 基因名称 | TPX2 |
| Gene ID (NCBI) | 22974 |
| RRID | AB_2208895 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9ULW0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TPX2 antibody 11741-1-AP | Download protocol |
| IHC protocol for TPX2 antibody 11741-1-AP | Download protocol |
| IF protocol for TPX2 antibody 11741-1-AP | Download protocol |
| IP protocol for TPX2 antibody 11741-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin. | ||
EMBO Rep Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis | ||
J Cell Sci Loss of protein phosphatase 6 in oocytes causes failure of meiosis II exit and impaired female fertility. | ||
J Cell Sci Importin α phosphorylation promotes TPX2 activation by GM130 to control astral microtubules and spindle orientation. | ||
Front Physiol Dysfunction of Sister Chromatids Separation Promotes Progression of Hepatocellular Carcinoma According to Analysis of Gene Expression Profiling. |
