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  • Featured Product
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TPX2 Polyclonal antibody

TPX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 11741-1-AP

Print datasheet

Synonyms

DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2

验证数据展示

  • WB analysis using 11741-1-AP

    WB analysis using 11741-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11741-1-AP (TPX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11741-1-AP

    IHC staining of human colon cancer using 11741-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPX2 antibody included
  • IHC staining of human prostate cancer using 11741-1-AP

    IHC staining of human prostate cancer using 11741-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11741-1-AP (TPX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPX2 antibody included
  • IHC staining of human colon cancer using 11741-1-AP

    IHC staining of human colon cancer using 11741-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPX2 antibody included
  • IP experiment of HeLa using 11741-1-AP

    IP experiment of HeLa using 11741-1-AP

    IP result of anti-TPX2 (IP:11741-1-AP, 4ug; Detection:11741-1-AP 1:800) with HeLa cells lysate 880ug.

  • IF Staining of HeLa using 11741-1-AP

    IF Staining of HeLa using 11741-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11741-1-AP (TPX2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 11741-1-AP

    IF Staining of HepG2 using 11741-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TPX2 antibody (11741-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, HeLa cells, SMMC-7721 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy TPX2 Ready-To-Use IHC Kit. TPX2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11741-1-AP targets TPX2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen TPX2 fusion protein Ag2334 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TPX2, microtubule-associated, homolog (Xenopus laevis)
Synonyms DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2
Calculated Molecular Weight 747 aa, 86 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC020207
Gene Symbol TPX2
Gene ID (NCBI) 22974
RRIDAB_2208895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULW0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).

实验方案

Product Specific Protocols
WB protocol for TPX2 antibody 11741-1-APDownload protocol
IHC protocol for TPX2 antibody 11741-1-APDownload protocol
IF protocol for TPX2 antibody 11741-1-APDownload protocol
IP protocol for TPX2 antibody 11741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

J Cell Biol

WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.

Authors - Junjie Huang
View Article
mouseWB,IF

EMBO Rep

Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis

Authors - Jia-Qian Ju
View Article
humanWB,IF

BMC Biol

CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment.

Authors - Haining Zhou
View Article
mouseWB

J Cell Sci

Loss of protein phosphatase 6 in oocytes causes failure of meiosis II exit and impaired female fertility.

Authors - Meng-Wen Hu
View Article
humanIF

J Cell Sci

Importin α phosphorylation promotes TPX2 activation by GM130 to control astral microtubules and spindle orientation.

Authors - Haijing Guo
View Article
humanIHC

Front Physiol

Dysfunction of Sister Chromatids Separation Promotes Progression of Hepatocellular Carcinoma According to Analysis of Gene Expression Profiling.

Authors - Baozhen Sun
View Article