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验证数据展示

  • IF Staining of HepG2 using CL488-11741

    IF Staining of HepG2 using CL488-11741

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TPX2 antibody (CL488-11741) at dilution of 1:200, CL594-Phalloidin (red).

  • IF Staining of HeLa using CL488-11741

    IF Staining of HeLa using CL488-11741

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TPX2 antibody (CL488-11741) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated TPX2 Polyclonal antibody

TPX2 Polyclonal Antibody for IF/ICC
Cat No. CL488-11741

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy TPX2 Ready-To-Use IHC Kit. TPX2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11741-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11741 targets TPX2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 TPX2 fusion protein Ag2334 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TPX2, microtubule-associated, homolog (Xenopus laevis)
别名 DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2
计算分子量 747 aa, 86 kDa
观测分子量 100 kDa
GenBank蛋白编号BC020207
基因名称 TPX2
Gene ID (NCBI) 22974
RRIDAB_3672510
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULW0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TPX2 antibody CL488-11741Download protocol
Standard Protocols
Click here to view our Standard Protocols