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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TPX2 Polyclonal antibody

TPX2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11741

Print datasheet

Synonyms

DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2

验证数据展示

  • IF Staining of HepG2 using CL488-11741

    IF Staining of HepG2 using CL488-11741

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TPX2 antibody (CL488-11741) at dilution of 1:200, CL594-Phalloidin (red).

  • IF Staining of HeLa using CL488-11741

    IF Staining of HeLa using CL488-11741

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TPX2 antibody (CL488-11741) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy TPX2 Ready-To-Use IHC Kit. TPX2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11741-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11741 targets TPX2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen TPX2 fusion protein Ag2334 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TPX2, microtubule-associated, homolog (Xenopus laevis)
Synonyms DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2
Calculated Molecular Weight 747 aa, 86 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC020207
Gene Symbol TPX2
Gene ID (NCBI) 22974
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULW0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TPX2 antibody CL488-11741Download protocol
Standard Protocols
Click here to view our Standard Protocols