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验证数据展示

  • WB analysis using 85118-6-RR (same clone as 85118-6-PBS)

    WB analysis using 85118-6-RR (same clone as 85118-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85118-6-RR (TPX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85118-6-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 85118-6-RR (same clone as 85118-6-PBS)

    IHC staining of human cervical cancer using 85118-6-RR (same clone as 85118-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85118-6-RR (TPX2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85118-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPX2 antibody included
  • IF Staining of HepG2 using 85118-6-RR (same clone as 85118-6-PBS)

    IF Staining of HepG2 using 85118-6-RR (same clone as 85118-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TPX2 antibody (85118-6-RR, Clone: 242528G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85118-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 85118-6-RR (same clone as 85118-6-PBS)

    FC experiment of HeLa using 85118-6-RR (same clone as 85118-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TPX2 Recombinant antibody (85118-6-RR, Clone:242528G6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85118-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85118-6-RR

    Affinity and Kinetic Characterization of 85118-6-RR

    Biolayer interferometry (BLl) kinetic assays of 85118-6-RR against Human TPX2 were performed. The affinity constant is 2.24 nM.

TPX2 Recombinant antibody, PBS Only

TPX2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85118-6-PBS

产品说明书

CloneNo. 242528G6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

242528G6, C20orf1, C20orf2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TPX2 Ready-To-Use IHC Kit. TPX2 primary antibody included.

产品信息

85118-6-PBS targets TPX2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 TPX2 fusion protein Ag2334 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TPX2, microtubule-associated, homolog (Xenopus laevis)
别名 242528G6, C20orf1, C20orf2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL 2
计算分子量 747 aa, 86 kDa
观测分子量 100 kDa
GenBank蛋白编号BC020207
基因名称 TPX2
Gene ID (NCBI) 22974
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9ULW0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).