TRAF2 Monoclonal antibody, PBS Only

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

2A4D1

Cat No : 67315-1-PBS

Synonyms

TRAF2, TRAP, TRAP3

WB analysis using 67315-1-Ig (same clone as 67315-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67315-1-Ig (TRAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67315-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67315-1-Ig (TRAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67315-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 67315-1-Ig (same clone as 67315-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF2 antibody (67315-1-Ig, Clone: 2A4D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67315-1-PBS in a different storage buffer formulation.

Size

67315-1-PBS targets TRAF2 in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications	WB,Indirect ELISA,IF Application Description
Tested Reactivity	Human
Immunogen	TRAF2 fusion protein Ag25387 种属同源性预测
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Full Name	TNF receptor-associated factor 2
Synonyms	TRAF2, TRAP, TRAP3
Calculated Molecular Weight	56 kDa
Observed Molecular Weight	53 kDa
GenBank Accession Number	BC032410
Gene Symbol	TRAF2
Gene ID (NCBI)	7186
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q12933
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.