TRAF2 Monoclonal antibody, PBS Only

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A4D1

Cat no : 67315-1-PBS

Synonyms

TRAF2, TRAP, TRAP3

WB analysis using 67315-1-Ig (same clone as 67315-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67315-1-Ig (TRAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67315-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67315-1-Ig (TRAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67315-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 67315-1-Ig (same clone as 67315-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF2 antibody (67315-1-Ig, Clone: 2A4D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67315-1-PBS in a different storage buffer formulation.

Size

67315-1-PBS targets TRAF2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications	WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity	Human
Immunogen	TRAF2 fusion protein Ag25387 种属同源性预测
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Full Name	TNF receptor-associated factor 2
Synonyms	TRAF2, TRAP, TRAP3
Calculated Molecular Weight	56 kDa
Observed Molecular Weight	53 kDa
GenBank Accession Number	BC032410
Gene Symbol	TRAF2
Gene ID (NCBI)	7186
RRID	AB_2882575
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q12933
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C