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TRAF2 Monoclonal antibody

TRAF2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2A4D1

Cat no : 67315-1-Ig

Print datasheet

Synonyms

TRAF2, TRAP, TRAP3

验证数据展示

  • WB analysis using 67315-1-Ig

    WB analysis using 67315-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67315-1-Ig (TRAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 67315-1-Ig

    IF Staining of HeLa using 67315-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF2 antibody (67315-1-Ig, Clone: 2A4D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, THP-1 cells, K-562 cells, Jurkat cells, L02 cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67315-1-Ig targets TRAF2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity Human
Cited Reactivityhuman
Immunogen TRAF2 fusion protein Ag25387 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name TNF receptor-associated factor 2
Synonyms TRAF2, TRAP, TRAP3
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC032410
Gene Symbol TRAF2
Gene ID (NCBI) 7186
RRIDAB_2882575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12933
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRAF2 antibody 67315-1-IgDownload protocol
IF protocol for TRAF2 antibody 67315-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cell Rep

Liquidambaric acid inhibits Wnt/β-catenin signaling and colon cancer via targeting TNF receptor-associated factor 2.

Authors - Rong Yan
View Article
humanWB,IP

FEBS J

TRAF2 associates with cullin neddylation complex assembly

Authors - Tiantian Wang
  • KO Validated
View Article