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CoraLite®594-conjugated TRAF2 Monoclonal antibody

TRAF2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A4D1

Cat no : CL594-67315

Print datasheet

Synonyms

TRAF2, TRAP, TRAP3

验证数据展示

  • IF Staining of HeLa using CL594-67315

    IF Staining of HeLa using CL594-67315

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 TRAF2 antibody (CL594-67315, Clone: 2A4D1 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-67315

    FC experiment of HeLa using CL594-67315

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TRAF2 (CL594-67315, Clone:2A4D1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67315-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67315 targets TRAF2 in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human
Immunogen TRAF2 fusion protein Ag25387 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name TNF receptor-associated factor 2
Synonyms TRAF2, TRAP, TRAP3
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC032410
Gene Symbol TRAF2
Gene ID (NCBI) 7186
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12933
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 TRAF2 antibody CL594-67315Download protocol
FC protocol for CL594 TRAF2 antibody CL594-67315Download protocol
Standard Protocols
Click here to view our Standard Protocols