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Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody, PBS Only

TRAIL/CD253 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241419D2

Cat no : 98139-1-PBS

Print datasheet

Synonyms

TNFSF10, TRAIL, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL

验证数据展示

  • FC experiment of human peripheral blood mononuclear cells using 98139-1-RR (same clone as 98139-1-PBS)

    FC experiment of human peripheral blood mononuclear cells using 98139-1-RR (same clone as 98139-1-PBS)

    1x10^6 untreated or IFN-alpha treated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98139-1-PBS in a different storage buffer formulation.

  • FC experiment of human peripheral blood mononuclear cells using 98139-1-RR (same clone as 98139-1-PBS)

    FC experiment of human peripheral blood mononuclear cells using 98139-1-RR (same clone as 98139-1-PBS)

    1x10^6 IFN-alpha treated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98139-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98139-1-RR

    Affinity and Kinetic Characterization of 98139-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98139-1-RR against Human TRAIL/CD253 were performed. The affinity constant is below 1 pM.

产品信息

98139-1-PBS targets TRAIL/CD253 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor (ligand) superfamily, member 10
Synonyms TNFSF10, TRAIL, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL
Calculated Molecular Weight 281 aa, 33 kDa
GenBank Accession NumberBC032722
Gene Symbol TRAIL
Gene ID (NCBI) 8743
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP50591
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.