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验证数据展示

  • FC experiment of human peripheral blood mononuclear cells using 98139-1-RR

    FC experiment of human peripheral blood mononuclear cells using 98139-1-RR

    1x10^6 untreated or IFN-alpha treated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • FC experiment of human peripheral blood mononuclear cells using 98139-1-RR

    FC experiment of human peripheral blood mononuclear cells using 98139-1-RR

    1x10^6 IFN-alpha treated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human peripheral blood mononuclear cells were surface stained with 0.25 ug Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (98139-1-RR, Clone: 241419D2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98139-1-RR

    Affinity and Kinetic Characterization of 98139-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98139-1-RR against Human TRAIL/CD253 were performed. The affinity constant is below 1 pM.

Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody

TRAIL/CD253 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98139-1-RR

产品说明书

CloneNo. 241419D2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

TNFSF10, TRAIL, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inIFN-alpha treated human peripheral blood mononuclear cells

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98139-1-RR targets TRAIL/CD253 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 10
别名 TNFSF10, TRAIL, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL
计算分子量 281 aa, 33 kDa
GenBank蛋白编号BC032722
基因名称 TRAIL
Gene ID (NCBI) 8743
RRIDAB_3672285
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP50591
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.

实验方案

Product Specific Protocols
FC protocol for TRAIL/CD253 antibody 98139-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols