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TRAPA/SSR1 Monoclonal antibody, PBS Only

TRAPA/SSR1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2D7

Cat no : 67925-1-PBS

Print datasheet

Synonyms

DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, SSR alpha, SSR1, TRAP alpha, TRAPA

验证数据展示

  • WB analysis using 67925-1-Ig (same clone as 67925-1-PBS)

    WB analysis using 67925-1-Ig (same clone as 67925-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

    IHC staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRAPA/SSR1 antibody included
  • IHC staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

    IHC staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRAPA/SSR1 antibody included
  • IF Staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

    IF Staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using TRAPA/SSR1 antibody (67925-1-Ig, Clone: 1F2D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TRAPA/SSR1 Ready-To-Use IHC Kit. TRAPA/SSR1 primary antibody included.

产品信息

67925-1-PBS targets TRAPA/SSR1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen TRAPA/SSR1 fusion protein Ag30832 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name signal sequence receptor, alpha
Synonyms DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, SSR alpha, SSR1, TRAP alpha, TRAPA
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC007710
Gene Symbol TRAPA
Gene ID (NCBI) 6745
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43307
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).