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TRAPA/SSR1 Monoclonal antibody

TRAPA/SSR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1F2D7

Cat no : 67925-1-Ig

Print datasheet

Synonyms

DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, SSR alpha, SSR1, TRAP alpha, TRAPA

验证数据展示

  • WB analysis using 67925-1-Ig

    WB analysis using 67925-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 67925-1-Ig

    IHC staining of human ovary tumor using 67925-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRAPA/SSR1 antibody included
  • IHC staining of human ovary tumor using 67925-1-Ig

    IHC staining of human ovary tumor using 67925-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRAPA/SSR1 antibody included
  • IF Staining of human ovary tumor using 67925-1-Ig

    IF Staining of human ovary tumor using 67925-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using TRAPA/SSR1 antibody (67925-1-Ig, Clone: 1F2D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman ovary tumor tissue
Planning an IHC experiment? We recommend our IHCeasy TRAPA/SSR1 Ready-To-Use IHC Kit. TRAPA/SSR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67925-1-Ig targets TRAPA/SSR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen TRAPA/SSR1 fusion protein Ag30832 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name signal sequence receptor, alpha
Synonyms DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, SSR alpha, SSR1, TRAP alpha, TRAPA
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC007710
Gene Symbol TRAPA
Gene ID (NCBI) 6745
RRIDAB_2918677
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43307
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

实验方案

Product Specific Protocols
WB protocol for TRAPA/SSR1 antibody 67925-1-IgDownload protocol
IHC protocol for TRAPA/SSR1 antibody 67925-1-IgDownload protocol
IF protocol for TRAPA/SSR1 antibody 67925-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols