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验证数据展示

  • IF Staining of human ovary tumor using CL488-67925

    IF Staining of human ovary tumor using CL488-67925

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 TRAPA/SSR1 antibody (CL488-67925, Clone: 1F2D7 ) at dilution of 1:200.

  • IF Staining of human ovary tumor using CL488-67925

    IF Staining of human ovary tumor using CL488-67925

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 TRAPA/SSR1 antibody (CL488-67925, Clone: 1F2D7 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated TRAPA/SSR1 Monoclonal antibody

TRAPA/SSR1 Monoclonal Antibody for IF-P
Cat No. CL488-67925

产品说明书

CloneNo. 1F2D7

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF-P

别名

DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, SSR alpha, SSR1, TRAP alpha, TRAPA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman ovary tumor tissue
Planning an IHC experiment? We recommend our IHCeasy TRAPA/SSR1 Ready-To-Use IHC Kit. TRAPA/SSR1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67925-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67925 targets TRAPA/SSR1 in IF-P applications and shows reactivity with Human samples.

经测试应用 IF-P Application Description
经测试反应性 Human
免疫原 TRAPA/SSR1 fusion protein Ag30832 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 signal sequence receptor, alpha
别名 DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, SSR alpha, SSR1, TRAP alpha, TRAPA
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC007710
基因名称 TRAPA
Gene ID (NCBI) 6745
RRIDAB_2934593
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP43307
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TRAPA/SSR1 antibody CL488-67925Download protocol
Standard Protocols
Click here to view our Standard Protocols