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CoraLite® Plus 488-conjugated TRAPA/SSR1 Monoclonal antibody

TRAPA/SSR1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F2D7

Cat no : CL488-67925

Print datasheet

Synonyms

DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, SSR alpha, SSR1, TRAP alpha, TRAPA

验证数据展示

  • IF Staining of human ovary tumor using CL488-67925

    IF Staining of human ovary tumor using CL488-67925

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 TRAPA/SSR1 antibody (CL488-67925, Clone: 1F2D7 ) at dilution of 1:200.

  • IF Staining of human ovary tumor using CL488-67925

    IF Staining of human ovary tumor using CL488-67925

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 TRAPA/SSR1 antibody (CL488-67925, Clone: 1F2D7 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman ovary tumor tissue
Planning an IHC experiment? We recommend our IHCeasy TRAPA/SSR1 Ready-To-Use IHC Kit. TRAPA/SSR1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67925-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67925 targets TRAPA/SSR1 in IF-P applications and shows reactivity with Human samples.

Tested Applications IF-P Application Description
Tested Reactivity Human
Immunogen TRAPA/SSR1 fusion protein Ag30832 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name signal sequence receptor, alpha
Synonyms DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, SSR alpha, SSR1, TRAP alpha, TRAPA
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC007710
Gene Symbol TRAPA
Gene ID (NCBI) 6745
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43307
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TRAPA/SSR1 antibody CL488-67925Download protocol
Standard Protocols
Click here to view our Standard Protocols