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验证数据展示

  • IF Staining of A431 using CL488-67171

    IF Staining of A431 using CL488-67171

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 TRIB2 antibody (CL488-67171, Clone: 1A10A6 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67171

    FC experiment of HeLa using CL488-67171

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TRIB2 (CL488-67171, Clone:1A10A6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated TRIB2 Monoclonal antibody

TRIB2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67171

产品说明书

CloneNo. 1A10A6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

TRB2, TRB 2, GS3955, FLJ57420, C5FW

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67171-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67171 targets TRIB2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 TRIB2 fusion protein Ag7501 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tribbles homolog 2 (Drosophila)
别名 TRB2, TRB 2, GS3955, FLJ57420, C5FW
计算分子量 39 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC002637
基因名称 TRIB2
Gene ID (NCBI) 28951
RRIDAB_2934525
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ92519
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TRIB2 antibody CL488-67171Download protocol
Standard Protocols
Click here to view our Standard Protocols