验证数据展示
产品信息
67136-1-PBS targets TRIM21 as part of a matched antibody pair:
MP50371-2: 67136-1-PBS capture and 67136-3-PBS detection (validated in Cytometric bead array)
MP50371-4: 67136-1-PBS capture and 67136-5-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
经测试应用 | WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | TRIM21 fusion protein Ag28377 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | tripartite motif-containing 21 |
别名 | RNF 81, RING finger protein 81, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM21, 52 kDa ribonucleoprotein autoantigen Ro/SS-A |
计算分子量 | 475 aa, 54 kDa |
观测分子量 | 42-54 kDa |
GenBank蛋白编号 | BC010861 |
基因名称 | TRIM21 |
Gene ID (NCBI) | 6737 |
RRID | AB_2882435 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P19474 |
储存缓冲液 | PBS only , pH 7.3 |
储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.